Subcategories

To study organs in isolation, certain requirements must be met. These include controlled temperature, a nutrient or buffer at physiological pH, aeration for the buffer, a perfusion pump to move the buffer and a system with a chamber to hold the isolated organ. Some organs may require additional equipment related to their specialized functions, such as a ventilator for isolated perfused lungs. Additionally, in order to ensure the health of the isolated organ, experimental conditions and various physiological parameters are monitored. These include perfusate flow and pressure, pH, pO2, pCO2, and biopotentials.

All functional isolated organ systems require the following components:

  • Base system for isolated heart, isolated lung or abdominal organ perfusion
  • Pump for perfusate (buffer) movement and control
  • Thermocirculator to control the system temperature
  • Buffer reservoirs and oxygenators to maintain temperature and aeration of the perfusate
  • Species and organ specific cannulae
  • Transducers and amplifiers for physiological measurements
  • Data acquisition and analysis method