The Manual Injector is a manual syringe driver for pneumatic or hydraulic control of injection needles or holding pipettes. It is suitable for injecting volumes in the nanoliter to microliter range. It is also widely used as a fluid control device for applications requiring sensitive manual control of the displacement of microneedle contents. The system is constructed from the highest quality parts. The base assembly is black anodized aluminum. A non-rotating Mitutoyo micrometer provides the drive to the gas-tight syringe1. A precision 3-way valve provides a convenient method for filling the fluid line and clearing air bubbles from the line. Teflon tubing, chromatography connectors and a pipette holder2 complete the system.
The injection resolution is dependent on the volume of the syringe that is installed, as outlined in the table below. For example, with a 25µl syringe installed, one complete rotation of the micrometer (25 divisions) yields a displacement equivalent to a volume of 267nl and turning the micrometer one division (0.001in) yields 10.6nl.
Syringe Volume |
Volume Per Revolution |
Volume Per Division |
---|---|---|
10µl | 106nl | 4.2nl |
25µl | 267nl | 10.6nl |
50µl | 529nl | 21.2nl |
100µl | 1.06µl | 42.3nl |
250µl | 2.65µl | 105.8nl |
500µl | 5.29µl | 211.7nl |
1000µl | 10.58µl | 423.4nl |