The Manual Injector is a manual syringe driver for pneumatic or hydraulic control of injection needles or holding pipettes. It is suitable for injecting volumes in the nanoliter to microliter range. It is also widely used as a fluid control device for applications requiring sensitive manual control of the displacement of microneedle contents. The system is constructed from the highest quality parts. The base assembly is black anodized aluminum. A non-rotating Mitutoyo micrometer provides the drive to the gas-tight syringe1. A precision 3-way valve provides a convenient method for filling the fluid line and clearing air bubbles from the line. Teflon tubing, chromatography connectors and a pipette holder2 complete the system.

The injection resolution is dependent on the volume of the syringe that is installed, as outlined in the table below. For example, with a 25µl syringe installed, one complete rotation of the micrometer (25 divisions) yields a displacement equivalent to a volume of 267nl and turning the micrometer one division (0.001in) yields 10.6nl.

 

Syringe
Volume
Volume Per
Revolution
Volume Per
Division
10µl 106nl 4.2nl
25µl 267nl 10.6nl
50µl 529nl 21.2nl
100µl 1.06µl 42.3nl
250µl 2.65µl 105.8nl
500µl 5.29µl 211.7nl
1000µl 10.58µl 423.4nl
Manual Microinjector